Biotechnologies

Biosolutions for every applications

High-Value Active Molecules, Microorganisms, Specialty Enzymes and Industrial Biocatalysis

SEQENS Biotechnologies offers biosolutions for every applications, including high-value active molecules, microorganisms, specialty enzymes and industrial biocatalysis. Within two cutting edge research centers in France, Protéus and Alganelle, we focus on developing bioproducts through proprietary projects and collaborations with expert partners.

>> Learn more about Alganelle by SEQENS
>> Learn more about Protéus by SEQENS

Technologies

Biotransformation
by Biocatalysis

Bioconversion
by in vivo cascade Biocatalysis

Precision Fermentation

Biosynthesis
Synthetic Biology & Metabolic Engineering

Microbiology
Isolation
Adaptative evolution

>>Learn more about our Biocatalysis

>>Learn more about our Synthetic Biology

Products

Specialty Enzymes
Natural or Optimized

High-Value Active Molecules
Bioactive Proteins, Polysaccharides, Oligosaccharides, Pigments, Vitamins

Microorganisms
Natural or Evolved (In)active strain, supernatant, cell extract, metabolites, proteins, peptides, enzymes

Markets

Flavors & Fragrances
Development of fully enzymatic pathways for natural label

Cosmetics
Development of COSMOS certified processes and high-value active molecules

Pharmaceuticals or Health
Development of enzymes for asymmetric synthesis and chiral resolution for pre-GMP or GMP ton-scale manufacturing

Food
Development of enzymatic tools used as synthesis auxiliaries

Agrisciences
Development of biocontrol, biostimulant and specialty enzyme solutions

Partnership R&D Organization

Partnerships

SEQENS Biotechnologies, stands as a PRDO (Partnership R&D organization) offering co-development opportunities on various markets and topics like food, cosmetics and pharmaceuticals.

One-off Services

Proof of concept, fermentation, biocatalysis process development or product manufacturing as fee-for-service model. Enzyme supply and strain licensing for tailor-made processes in Food and Fine-chemicals markets.

Proprietary Products

We market off-the-shelf products.

>>Check our products

SEQENS biotechnological platforms

SEQENZYM

SEQENSYNT

SEQENBIOTICS

Enzyme screening

Enzyme collection
Proprietary (meta)genomic data
In-silico selection

Enzyme engineering (BIOCATALYSIS)

Bioinformatics
Directed Evolution

Strain engineering (BIOCONVERSION)

Metabolic engineering

Biocatalytic Process

Fermentation Recombinant enzyme production

Precision fermentation In vivo transformation of substrate

Enzyme production

Target ingredient manufacturing (GMP, non-GMP & non-pharma) Kg to Tons

Strain contruction & selection

Genetic & Metabolic engineering
Various cell factories: Bacteria, Yeast, Cyanobacteria, Microalgae

Strain engineering (SYNTHETIC BIOLOGY)

Growth & productivity optimization

Precision fermentation

Bioinspired ingredient bioproduction optimization

Target ingredient manufacturing (GMP, non-GMP & non-pharma) Kg to Tons

Strain screening

for metabolites of interest
Library >5000 strains
New strain isolation

Strain optimization (MICROBIOLOGY)

Natural adaptative evolution
or Metabolic engineered

Fermentation

Natural product

Target ingredient manufacturing

Application Market

PHARMA APIs SYNTHESIS

SEQENZYM VL API multi-tons biosynthesis on Seqens manufacturing site

Enzyme production scale-up from 40L to 300L
Biocatalyst loading 0.2%
Enzyme cost/kg product is below <5%
High specificity ee >99.9%
High productivity 200g/L
High level of reproducibility

PHARMA APIs SYNTHESIS

SEQENZYM® AL D-Serine multi-tons biosynthesis on Seqens manufacturing site

Enzyme production scale-up from 300L to 15m3
High specificity ee >99.9%
High substrate concentration >30%
Enzyme cost/kg product is below <5%

FOOD & FEED

SEQENZYM® FT Bioconversion of sucrose into short-chain Fructo-Oligosaccharides (FOS) Benefits of SEQENZYM® FT

Independant from sugar source
Highest yield > 60% Total FOS
Higher purification yields
Lowest free Fructose < 1%
No color formation < 10 ICUMSA

COSMETICS

Biocatalytic route to Estolides a high naturality process for a plant-based ingredient

Biosourced substrates from castor oil (non food crop)
Biosynthetic process advantages: Low temperature; No color; Lower energy consumption; No metal catalyst
Variable properties: Solid, Liquid, Semi-solid; Emulsion, depends on capping agents; Prolonged release; Well tolerated; GRAS: Oral and topical administration
Cosmetic applications: Skin hydration, Natural antioxydant, Hair additive, Texturizing

FLAVOR & FRAGRANCE

(-)-Ambrox biosynthesis Squalene hopene cyclase optimization

1x round of directed evolution
Optimization of the reaction conditions by DoE for the best performing variant
Factor 10x productivity improvement
Conversion obtained with 125g/L of substrate with the best variant

« Biocatalytic Process for (−)-Ambrox Production Using Squalene Hopene Cyclase ». Source: Advanced Synthesis & Catalysis 360, no 12 (15 juin 2018): 2339‑51 >>Click to read the corresponding publication

FLAVOR & FRAGRANCE

Green Notes biosynthesis Optimization of a 13-HPL from guava puree

4x round of directed evolution with homologous enzymes
65,000 variants screened
Factor 15x activity improvement
Factor 48x thermostability improvement

AGROSCIENCES

Biocontrol Toxin Discovery Collaborative Project DIETETIC

Identification of insecticidal activities against corn rootworm larvae (Diabrotica virgifera virgifera) from Protéus microbial diversity
1’300 strains screened
Co-development opportunities